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Optimization of Moringa oleifera leaf extraction and investigation of anti-breast cancer activity with the leaf extract

Hossain, Nazia and Mirghani, Mohamed Elwathig Saeed and Raus, Raha Bt. Ahmad (2015) Optimization of Moringa oleifera leaf extraction and investigation of anti-breast cancer activity with the leaf extract. Engineering International, 3 (2). pp. 97-103. ISSN 2409-3629

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Abstract

Moringa oleifera (M. oleifera) has been considered as medicinal plant and food substance since ancient times. Nowadays various therapeutic effects of M. oleifera such as antimicrobial, anticancer, anti-inflammatory, antidiabetic, and antioxidant effects have been successfully investigated. In this study, the main objective was to optimize M. oleifera leaf-methanol extraction using Design of Experiment (DoE) software with response surface methodology in terms of temperature and incubation time by sonication. The other objective was to investigate anti-cancer activity (breast cancer cell: MCF-7) by M. oleifera methanol extraction incorporation with microtitrate tetrazolium (MTT) assay. In this research, the optimum condition for leaf extraction was found to be temperature 50⁰ C and incubation time 45 min with medium frequency at sonication and it obtained 88.39% cancer cell growth inhibition by this condition. Extracted leaves inhibited MCF-7 cell line with 87.13% in average at wavelength A570nm.

Item Type: Article (Journal)
Additional Information: 4971/50116
Uncontrolled Keywords: Moringa oleifera leaves extract, Breast Cancer Inhibition, Anti-cancer Activity, Optimization, Response Surface Methodology
Subjects: Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Assoc Prof Elwathig Mirghani
Date Deposited: 27 Apr 2016 11:51
Last Modified: 26 Feb 2018 19:22
URI: http://irep.iium.edu.my/id/eprint/50116

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