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Single-step bioconversion of starch to bioethanol by the coculture of ragi tapai and saccharomyces cerevisiae

Azmi, Azlin Suhaida and Hasan, Masitah and Mel, Maizirwan and Ngoh, Cheng (2009) Single-step bioconversion of starch to bioethanol by the coculture of ragi tapai and saccharomyces cerevisiae. Chemical Engineering Transactions, 18. pp. 557-562. ISSN 1974-9791

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Abstract

The objective of this preliminary experiment was to test the hypothesis that coculturing commercialised ragi tapai with S. cerevisiae might have improved the ethanol production and reduce the accumulation of inhibitory concentration of reducing sugar and this would enhance the amylolytic activity. Co culture of a commercialised ragi tapai with S cerevisiae using unhydrolysed raw starch in single step-fermentation produced a high ethanol concentration. A 35.26 giL of ethanol was produced when the starch was inoculated with ragi tapai and cocultured it with Scerevisiae 1 h later. This was 48% higher than the yield obtained when the starch was inoculated with ragi tapai only (23.79 giL). The glucose concentration was maintained at low concentration in the coculture medium compared to the medium with ragi tapai only. However, stachyose concentration profile was higher in cocultured medium as compared to the one with ragi tapai only. In conclusion, coculture ragi tapai with S cerevisiae enhanced the ethanol production and prevented the inhibitory effect of reducing sugars on amylase activity.

Item Type: Article (Journal)
Additional Information: 5339/5001
Uncontrolled Keywords: Cassava starch; bioethanol; glucose; ragi tapai; Saccharomyces cerevisiae, single-step bioconversion
Subjects: T Technology > TP Chemical technology > TP155 Chemical engineering
T Technology > TP Chemical technology > TP248.13 Biotechnology
T Technology > TP Chemical technology > TP315 Fuel
T Technology > TP Chemical technology > TP500 Fermentation industries. Beverages. Alcohol
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering
Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr Maizirwan Mel
Date Deposited: 20 Sep 2011 15:53
Last Modified: 14 Jun 2017 09:19
URI: http://irep.iium.edu.my/id/eprint/5001

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