Optimization of lipase immobilization on nylon -6-grafted with PGMA polymer

Bahrudin, Nik Adlin and Al-Khatib, Ma An Fahmi Rashid and Nasef, Mohamed Mahmoud (2016) Optimization of lipase immobilization on nylon -6-grafted with PGMA polymer. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur.

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Abstract

Lipase from wheat germ was immobilized by covalent binding on a nylon -6- grafted with PGMA. This polymer was successfully activated with diethyl amine to intact with lipase enzyme molecule. Response surface methodology (RSM) was applied to model and optimize immobilization conditions for maximum activity and understanding the interaction of the factors affecting the activity of the immobilized enzyme. The face centered central composite design (FCCCD) was applied to measure the effect of reaction pH (6-8), reaction time (2-10 h) and enzyme concentration (1-2 mg) on enzyme activity. The result showed that pH, time and enzyme concentration had significant effects on the activity of lipase enzyme. Based on the analysis, the optimum immobilization conditions obtained were at pH 7, immobilization time 5 hours and enzyme concentration 0.90 mg/ml.

Item Type:	Conference or Workshop Item (Plenary Papers)
Additional Information:	5595/47871 (ISBN: 978-983-42978-8-6)
Uncontrolled Keywords:	Immobilization, lipase, nylon-6-grafted with PGMA, optimization, RSM.
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr. Ma'an Alkhatib
Date Deposited:	09 Aug 2016 14:28
Last Modified:	17 Jan 2017 09:42
URI:	http://irep.iium.edu.my/id/eprint/47871

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