Electrochemical sensor centrifuge platform for single-cell study

Wan Salim, Wan Wardatul Amani (2015) Electrochemical sensor centrifuge platform for single-cell study. In: 12th Asian Congress on Biotechnology (ACB 2015) , 15-19 November 2015, Hotel Istana, Kuala Lumpur.

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Abstract

SporeSat – a lab-on-a-chip (LOC) centrifuge platform designed for integration as the payload of a small (5.5 kg), free-flying satellite – has been developed to determine the gravitational thresholds for calcium-ion channel activation of a single-cell spore from the fern Ceratopteris richardii. This fern is an important model system for gravity-directed plant-cell development during variable-gravity conditions attainable only in space flight. Calcium-ion channel activity is measured by photolithographically defined calcium ion–selective electrodes (ISEs) at opposite ends of each spore. Artificial gravity is created by rotating a disk-like platform that contains the spores in wells along with the calcium ISEs. Ground experiments reveal a maximum calcium concentration ratio at 2.2xg, between micro-ion-selective electrodes near the “top” and “bottom” ends of the spore, indicating an increasing calcium concentration at one “end” of the fern spore with respect to the other. Confocal micrographs of rhizoid formation confirm the light-induced germination. SporeSat is a spaceflight experiment that will take ~ 4 days; data will be telemetered to Earth over ~ 100 days.

Item Type:	Conference or Workshop Item (Speech/Talk)
Additional Information:	7116/47224
Uncontrolled Keywords:	SporeSat, centrifuge, calcium-ion, ion-selective electrodes, artificial gravity, spaceflight, free-flying satellite
Subjects:	S Agriculture > S Agriculture (General) T Technology > TP Chemical technology > TP248.13 Biotechnology T Technology > TP Chemical technology > TP250 Industrial electrochemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr Wan Wardatul Amani Wan Salim
Date Deposited:	04 Feb 2016 15:50
Last Modified:	20 May 2016 14:29
URI:	http://irep.iium.edu.my/id/eprint/47224

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