Isolation of thermotolerant bacteria producing fibrinolytic enzyme

Ab Shukor, Nurul Aqilah and Amid, Azura and Abdul Karim, Mohamed Ismail and Abd Samad, Nadiah Syuhada (2015) Isolation of thermotolerant bacteria producing fibrinolytic enzyme. Jurnal Teknologi, 77 (25). pp. 83-87. ISSN 0127-9696 E-ISSN 2180-3722

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Abstract

Fibrinolytic enzymes were widely used in the treatment of cardiovascular diseases. However, the efficiency of the commercial enzymes are still lack of perfection because there are many side effects as well as not tolerant to downstream processing such as heat sensitive during spray drying process. Therefore, this study presents newly isolated thermophiles bacteria producing fibrinolytic enzyme. Sample was collected from Hot Spring Selayang at Selayang Selangor. Spread plate agar containing skim milk powder growth at pH 7, 53℃ for 24 hours was utilized to isolate thermotolerant bacteria producing protease. Further isolation on bacteria producing fibrinolytic enzyme was carried out using fibrin plate. 16S rDNA gene sequence analysis was used to identify the genotype of the isolates. 27 colonies of thermotolerant bacteria were isolated, however, only 19 of them showing proteolytic activity. All of the 19 isolates are motile and cocci in shapes, with 4 types of arrangement, which are single, diplo (pair), strepto (chain) and staphylo (cluster). HSP04 and HSP11 are gram positive bacteria and others are gram negative. From 19 isolates only 6 were chosen for further analysis. HSP23 showed the highest fibrinolytic activity compared with others. HSP23 was identified as Bacillus licheniformis with 98 % similarity to Bacillus licheniformis DCM 13 and Bacillus licheniformis strain ATCC 14580.

Item Type:	Article (Journal)
Additional Information:	3688/47010
Uncontrolled Keywords:	fibrinolytic enzyme, thermotolerant bacteria, bacteria identification
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Prof Ts Dr Azura Amid
Date Deposited:	01 Jan 2016 14:47
Last Modified:	27 Sep 2017 11:27
URI:	http://irep.iium.edu.my/id/eprint/47010

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