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Identification and verification of porcine DNA in commercial gelatin and gelatin containing processed foods

Abdullah Amqizal, Hassan Ibrahem and Al-Kahtani, Hassan Abdullah M. and Ismail, Elsayed Ali and Hayat, Khizar and Jaswir, Irwandi (2017) Identification and verification of porcine DNA in commercial gelatin and gelatin containing processed foods. Food Control, 78. pp. 297-303. E-ISSN 0956-7135

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Abstract

Gelatin, derived from bovine and porcine sources, has been used in many foods and pharmaceutical products. To ensure the compliance of food products with halal regulations, the reliable analytical methods are very much required. In this study, polymerase chain reaction (PCR) assay using species-specific primers was performed to evaluate the halal authenticity of commercial pure gelatin and gelatin-containing processed food products. Based on the specificity and cross-reactivity results of the seven species-specific primers by conventional PCR, the porcine species primer No. 2 was selected and it was able to detect species DNA in 12 out of 36 processed foods. The cloning, sequencing, and blasting at NCBI confirmed the presence of pork DNA in 5 out of 12 porcine DNA positive food samples. The maximum identity (homology) with pork sequence available in NCBI Gene Bank for the five samples ranged from 87% to 97% and the Query Cover ranged from 94% to 100%. The real-time PCR assay detected more positive samples (27 positive amplifications) compared to 12 positive samples with conventional PCR using porcine specific primer No. 2. PCR using species specific primers is a very useful and effective technique for halal authenticity of gelatin and gelatin-containing food products.

Item Type: Article (Journal)
Additional Information: 5784/46727
Uncontrolled Keywords: Halal authenticity; PCR; Gelatin; Processed foods; Species-specific primers
Subjects: T Technology > TP Chemical technology > TP368 Food processing and manufacture
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering
Depositing User: Faizah Shahbudin
Date Deposited: 06 Jul 2017 16:24
Last Modified: 06 Jul 2017 16:24
URI: http://irep.iium.edu.my/id/eprint/46727

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