Cytotoxicity studies of lung cancer cells using impedance biosensor

Mohd Mansor, Ahmad Fairuzabadi and Nordin, Anis Nurashikin and Ibrahim, Irmanisha (2015) Cytotoxicity studies of lung cancer cells using impedance biosensor. In: 2015 International Conference on Smart Sensors and Application (ICSSA), 26th-28th May 2015, Grand Seasons Hotel, Kuala Lumpur.

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Abstract

Electrical cell-substrate impedance sensing (ECIS) is a valuable tool for real time monitoring of cell behavior such as attachment, mobility, and growth. To employ ECIS, the cells need to attach, spread and proliferate on the sensor in the presence of adhesion-promoting protein that mimics the extracellular matrix (ECM) of the cells. For cell attachment, collagen I, Bovine had been used as the coating substrate. In this study, four designs with varying electrode distances had been measured to detect the changes in impedance values of Lung Carcinoma cell lines (A549). The impedance change due to the cell growth and attachment was modeled as an equivalent circuit consisting of resistors and capacitors of both the cell culture media and the cells. The impedance measurements were measured every 8 hours for 120 hours at frequencies of 100Hz to 10MHz using Agilent Precision Impedance Analyzer 4294A. The experimental results have shown that the closest distance of the electrode gave the most optimum impedance value for A549 cancer cell’s measurement. The cancer cells were also treated with a chemotherapeutic drug, Taxol and its impedance response was monitored over 5 days. Experimental results show that there is significant reduction in impedance when the cancer cells were exposed to Taxol, indicating that the cells are no longer adherent to the sensor’s surface or are dead.

Item Type:	Conference or Workshop Item (Plenary Papers)
Additional Information:	4575/46640
Uncontrolled Keywords:	ECIS; A549; Cell Adhesion; Collagen,; interdigitated Circuit;
Subjects:	T Technology > TK Electrical engineering. Electronics Nuclear engineering > TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Electrical and Computer Engineering Kulliyyah of Engineering
Depositing User:	Dr. Anis Nurashikin Nordin
Date Deposited:	07 Jan 2016 16:21
Last Modified:	05 Sep 2019 11:16
URI:	http://irep.iium.edu.my/id/eprint/46640

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