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Potential thermostable mutants of endoglucanase I from fusarium oxysporum

Ibrahim Ali , Noorbatcha and Hamzah, Mohd. Salleh (2015) Potential thermostable mutants of endoglucanase I from fusarium oxysporum. In: 5th International Conference on the Advancement in Science and Technology (ICAST 2015), 10-12 AUGUST 2015 , Impiana Resort Cherating, Pahang MALAYSIA. (Unpublished)

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Abstract

Lignocellulose is the most abundant biopolymer on earth and it is a good renewable energy source. Thermostable enzymes have several benefits in various industrial applications. Among the benefits are they allow the use of increased substrate concentration in the reaction mixture, as the viscosity will drop at higher temperature which eventually will improve product yield and reduces capital and processing costs. This research intends to predict computationally, and develop experimentally, a thermostable mutant endoglucanase that can operate at higher temperature as required by industrial processes. Our preliminary computational data based on rational design approach suggest that quintuple mutant, T224E/G229A/S230F/S231E/N321R, of an endoglucanase from a pathogenic fungus, Fusarium oxysporum, can potentially remain active at higher temperature (80oC) compared to its current operating temperature (<60oC). In our effort to experimentally produce this quintuple mutant we have successfully produced a single mutant, T224E, and a triple mutant, G229A/S230F/S231E, via site directed mutagenesis. We are reporting here the characterization results of these two mutants as compared to the wild type. Keywords: Thermostable enzymes, mutant endoglucanases, Fusarium oxysporum

Item Type: Conference or Workshop Item (Slide Presentation)
Additional Information: 3704/46338
Uncontrolled Keywords: thermostable mutants, endoglucanase I, fusarium oxysporum
Subjects: T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering
Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Prof. Dr. Ibrahim Ali Noorbatcha
Date Deposited: 06 Jan 2016 15:36
Last Modified: 06 Jan 2016 15:39
URI: http://irep.iium.edu.my/id/eprint/46338

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