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Functional characterization of recombinant bromelain

Amid, Azura and Bala, Muntari and Mohd. Salleh, Hamzah (2015) Functional characterization of recombinant bromelain. In: ASIAN Congress on Biotechnology 2015, 15th-19th Nov. 2015, Kuala Lumpur.

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Abstract

Stem bromelain is a plant protease with a number of industrial and therapeutic applications. This study investigated the functional properties of commercial bromelain and recombinant bromelain (expressed in E. coli BL 21-AI). In vitro cytotoxicity assays were conducted to evaluate the effect of both bromelains on tumour cell lines (murine melanoma and breast cancer MCF-7) and normal cells (Vero and Chinese hamster ovary) using Sulforhodamine B and MTT tests. Anti-inflammatory activities of the enzymes were also assessed on murine macrophage cell lines using Griess reagents and ELISA test. The beef tenderizing effects of both bromelains and in-situ localization of the recombinant bromelain were analysed using Transmission Electron Microscopy. The results obtained revealed that both bromelains were more cytotoxic to tumour cell lines (IC50 values ≈ 0.16-0.23mg/mL) than normal cell lines (IC50 values ≈ 0.27-0.51mg/mL). Moreover, the two enzymes had effectively inhibited the pro-inflammatory mediators’ production and thus, had good anti-inflammatory activity. Both enzymes were found to have comparative anti-cancer and anti-inflammatory properties. The expression of recombinant enzyme was discovered to occur in the cytoplasm of E. coli BL 21-AI and the purified enzyme efficiently degraded both actin and myosin of fresh beef. Hence, it served as a good meat tenderizer.

Item Type: Conference or Workshop Item (Speech/Talk)
Additional Information: 3688/45893
Uncontrolled Keywords: anti-inflammatory, bromelain, cytotoxicity, subcellular, tenderizing
Subjects: T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr Azura Amid
Date Deposited: 30 Nov 2015 11:18
Last Modified: 23 May 2016 10:10
URI: http://irep.iium.edu.my/id/eprint/45893

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