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Characterization of valuable compounds from winter melon (benincasa hispida (thunb.) Cogn.) seeds using supercritical carbon dioxide extraction combined with pressure swing technique

Bimakr, Mandana and Abdul Rahman, Russly and Ganjloo, Ali and Taip, Farah Saleena and Mohd Adzahan, Noranizan and Sarker, Md. Zaidul Islam (2016) Characterization of valuable compounds from winter melon (benincasa hispida (thunb.) Cogn.) seeds using supercritical carbon dioxide extraction combined with pressure swing technique. Food and Bioprocess Technology, 9 (3). pp. 396-406. ISSN 1935-5130 E-ISSN 1935-5149

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Abstract

In this study, we describe the extraction of different valuable compounds from winter melon seeds using supercritical carbon dioxide extraction combined with pressure swing technique (SCE-PST). The effects of the extraction variables,namely pressure, holding time (HT), and continuous extraction time (CT), were optimized by response surface methodology (RSM) to maximize the crude extraction yield (CEY). The optimal conditions were at pressure of 181.35 bar, HT of 9.93 min, and CT of 50.14 min. Under these conditions, the experimental CEY was 235.70±0.11 mg g−1 with a relatively strong antioxidant activity (64.42±0.21 % inhibition of DPPH· radicals, 67.36±0.34 % inhibition of ABTS·+ radicals) and considerable amount of phenolic compounds (42.77±0.40 mg gallic acid equivalent/g extract). The high-performance liquid chromatography (HPLC) analysis revealed that the bioactive phenolic compounds increased significantly using PST (p<0.05), where gallic acid had the highest concentration (0.688±0.34 mg g−1 ). The extract obtained using optimal SCE-PST conditions contained more than 83.65 % total unsaturated fatty acids (UFAs) and linoleic acid accounted for 67.33±0.22 % in the total extract. From the results, the SCE efficiency in terms of extract quantity and quality has been enhanced significantly applying PST. Finally, the results were compared with previous published findings using supercritical carbon dioxide, ultrasoundassisted, and Soxhlet extraction. It was found that higher CEY could be achieved using Soxhlet extraction even through the quality of SCE-PST extracts in terms of antioxidant activity and phenolic compounds was better.

Item Type: Article (Journal)
Additional Information: 6605/45681
Uncontrolled Keywords: Winter melon . SCE-PST . Antioxidant activity . Phenolic compounds . HPLC . UFA
Subjects: Q Science > Q Science (General)
T Technology > T Technology (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User: Professor Dr. Md. Zaidul Islam Sarker
Date Deposited: 16 Nov 2015 08:43
Last Modified: 05 Apr 2017 09:29
URI: http://irep.iium.edu.my/id/eprint/45681

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