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Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh

Shahidan, Norshazila and Othman, Rashidi and Jaswir, Irwandi and Hashim, Yumi Zuhanis Has-Yun (2011) Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, The Legend Hotel, Kuala Lumpur.

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Abstract

Characterization and quantification of carotenoids composition is complicated, costly and time-consuming. The accuracy and the reliability of the data depend solely on the standards and HPLC but a major constraint is acquiring and maintaining pure standards. Carotenoid standards are commercially available but they are expensive and are prone to isomerization and oxidation. Thus the purpose of this study is to establish analytical method for isolating beta-carotene by open column chromatography from pumpkin or Cucurbita moschata to be used as one of the carotenoid standards for determination of total and individual carotenoids. Pumpkin with orange flesh has been chosen due to the non-seasonal and abundance all year round. The study on the scheme of obtaining beta-carotene standard from pumpkin flesh demonstrated that the purity of beta-carotene standard evaluated by HPLC was 97.95%. The standard curves with five different concentrations for betacarotene in triplicate were constructed by plotting the area against the concentration. The coefficient of correlation was 0.9990. This study established that pumpkin can be a reliable source of beta-carotene standard which is cheap and can provide a constant supply throughout the year.

Item Type: Conference or Workshop Item (Full Paper)
Additional Information: 3842/4562 (Proceeding of the 2nd International Conference on Biotechnology Engineering (ICBioE 2011))
Uncontrolled Keywords: caretenoids, ß-carotene, HLPC, Cucurbita moschata
Subjects: Q Science > Q Science (General)
Q Science > QD Chemistry
S Agriculture > S Agriculture (General)
S Agriculture > SB Plant culture
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Architecture and Environmental Design > Department of Landscape Architecture
Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr rashidi othman
Date Deposited: 04 Oct 2011 13:16
Last Modified: 19 Jan 2012 12:01
URI: http://irep.iium.edu.my/id/eprint/4562

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