Isolation of secondary metabolites from quercus infectoria galls and their antimicrobial activity

Sulaiman, Fatimatul Akma and Darnis, Deny Susanti and Mohamed Iqbal, Mohammad Anwar and Miskam, Mazidatulakmam Akmam (2015) Isolation of secondary metabolites from quercus infectoria galls and their antimicrobial activity. In: 5th International Conference on Advancement in Science and Technology (iCAST 2015), 10-12 August 2015, Impiana Resort Cherating. (Unpublished)

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Abstract

The evolution of resistant microorganism towards available antibiotics has gain interest among scientists to search for more effective antimicrobial compounds. Plant is believe to have antimicrobial properties as the extract is used in food industry, pharmaceuticals and alternative medicine. The aims of this study are to isolate secondary metabolites from Q. infectoria galls and to elucidate structure of the isolated compound using spectroscopic techniques and determination of antimicrobial activity. The galls were extracted with organic solvent using soxhlet apparatus and fractionated using chromatographic techniques. The isolated compounds were characterized using spectroscopic techniques such as UV spectrophotometer, Fourier Transform Infrared Spectroscopy (FTIR), Nuclear Magnetic Resonance (NMR) and Mass Spectrometry (MS). The antimicrobial activities are determined using disc diffusion method, determination of MIC, MBC, MFC and synergistic effect. The disc diffusion test against Staphylococcus aureus showed zone of inhibition 7 mm (n-hexane extract) and 9 mm (methanol extract). Meanwhile, dichloromethane extract showed no inhibition against S. aureus. It is expected that the isolated compound have antimicrobial properties and synergistic effect in combination with standard antibiotic.

Item Type:	Conference or Workshop Item (Poster)
Additional Information:	5641/44394
Uncontrolled Keywords:	Quercus infectoria; natural products; extraction; isolation; antimicrobial activity.
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Chemistry
Depositing User:	Dr Deny Susanti Darnis
Date Deposited:	26 Aug 2015 09:33
Last Modified:	22 May 2018 11:44
URI:	http://irep.iium.edu.my/id/eprint/44394

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