Identification of angiotensin I converting enzyme inhibitory and radical scavenging bioactive peptides from sea cucumber (Stichopus vastus) collagen hydrolysates through optimization

Abedin, Md. Zainul and Karim, A. A. and Gan, Chee-Yuen and Ghazali, F. C. and Barzideh, Z. and Zzaman, Wahidu and Sarker, Md. Zaidul Islam (2015) Identification of angiotensin I converting enzyme inhibitory and radical scavenging bioactive peptides from sea cucumber (Stichopus vastus) collagen hydrolysates through optimization. International Food Research Journal, 22 (3). pp. 1074-1082. ISSN 19854668 (P), 22317546 (O)

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Abstract

The sea cucumber (Stichopus vastus) is an underutilized species, as most of its parts, including the integument (high collagen content) are thrown away during processing. The aim of this study was to investigate the effects of different hydrolysis conditions (substrate to enzyme ratio (S/E), reaction temperature, and hydrolysis time) on the angiotensin I converting enzyme (ACE) inhibitory and radical scavenging (RSc) activities of the hydrolysates produced from trypsin hydrolysis of S. vastus collagen. Optimal conditions predicted by Box-Behnken Design modelling for producing ACE inhibitory and RSc hydrolysates were found to be S/E ratio (15), reaction temperature (55°C), and hydrolysis time (1 h). Under optimal conditions, ACE inhibitory and RSc activities were estimated to be as high as 67.8% and 77.9%, respectively. Besides, some novel bioactive peptides were identified through mass spectrometry analysis. These results indicate that S. vastus hydrolysates might be used as a functional ingredient in food and nutraceutical products.

Item Type:	Article (Journal)
Additional Information:	6605/43605
Uncontrolled Keywords:	Sea cucumber collagen, hydrolysates, Optimization, ACE inhibitory activity, RSc activity, Bioactive peptides
Subjects:	Q Science > Q Science (General) T Technology > T Technology (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User:	Professor Md. Zaidul Islam Sarker
Date Deposited:	30 Jun 2015 10:19
Last Modified:	03 Jul 2015 16:09
URI:	http://irep.iium.edu.my/id/eprint/43605

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