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A novel liquid/liquid extraction process composed of surfactant and acetonitrile for purification of polygalacturonase enzyme from durio zibethinus

Amid, Mehrnoush and Manap, Yazid and Azmira, Farhana and Hussina, Muhaini and Sarker, Md. Zaidul Islam (2015) A novel liquid/liquid extraction process composed of surfactant and acetonitrile for purification of polygalacturonase enzyme from durio zibethinus. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 993-94. pp. 1-8. ISSN 1570-0232

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Abstract

Polygalacturonase is one of the important enzymes used in various industries such as food, detergent, pharmaceutical, textile, pulp and paper. A novel liquid/liquid extraction process composed of surfactant and acetonitrile was employed for the first time to purify polygalacturonase from Durio zibethinus. The influences of different parameters such as type and concentration of surfactants, concentrations of acetonitrile and composition of surfactant/acetonitrile on partitioning behavior and recovery of polygalacturonase was investigated. Moreover, the effect of pH of system and crude load on purification fold and yield of purified polygalacturonase were studied. The results of the experiment indicated the polygalacturonase was partitioned into surfactant top rich phase with impurities being partitioned into acetonitrile bottom rich phase in the novel method of liquid/liquid process composed of 23% (w/w) Triton X-100 and 19% (w/w) acetonitrile, at 55.6% of TLL (tie line length) crude load of 25% (w/w) at pH 6.0. Recovery and recycling of components also was measured in each successive step of liquid/liquid extraction process. The enzyme was successfully recovered by the method with a high purification factor of 14.3 and yield of 97.3% while phase components were also recovered and recycled above 95%. This study demonstrated that the novel method of liquid/liquid extraction process can be used as an efficient and economical extraction method rather than the traditional methods of extraction for the purification and recovery of the valuable enzyme.

Item Type: Article (Journal)
Additional Information: 6605/43434
Uncontrolled Keywords: Novel liquid/liquid extraction process; Surfactant; Acetonitrile; Recycling of phase components; Polygalacturonase; Durio zibethinus
Subjects: Q Science > Q Science (General)
T Technology > T Technology (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User: Professor Dr. Md. Zaidul Islam Sarker
Date Deposited: 22 Jun 2015 11:22
Last Modified: 03 Nov 2017 12:39
URI: http://irep.iium.edu.my/id/eprint/43434

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