Development and validation of gas chromatography-mass spectrometric method for the determination of epoxidized soybean oil in foods stored in glass jars with metal lids.

Quek, Hong Keat and Wan Ibrahim, Wan Aini and Sanagi, Mohd Marsin and Miskam, Mazidatulakmam Akmam and Salleh, Syairah (2014) Development and validation of gas chromatography-mass spectrometric method for the determination of epoxidized soybean oil in foods stored in glass jars with metal lids. Sains Malaysiana, 43 (3). pp. 407-413. ISSN 0126-6039

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Abstract

A method has been developed for the determination of epoxidized soybean oil (ESBO) in oily foods stored in glass jars with metal lids using gas chromatography-mass spectrometry (GC-MS). ESBO and its internal standard (cis,cis-11,12;14,15- diepoxyeicosanoate) were isolated from the matrix by transesterification process. The developed method showed good linear dynamic range between 0.7-20 µg mL-1 with coefficients of determination (R2 ) > 0.9968 and acceptable limit of detection and limit of quantitation of 7 and 23 mg kg-1, respectively, based on linearity calculations. Analyte recoveries were 90.84±27.24% for low concentration, 78.05±11.59% for medium concentration and 99.23±10.20% for high concentration. This first fully validated GC-MS method was successfully applied for the determination of ESBO in foods stored in glass jar with metal lid. Among the 31 food samples studied, 6 samples were found to exceed the specific migration limit of 60 mg kg-1 (based on EU Directive 2002/72/EC). The developed method is thus potentially useful for routine analysis for the determination of ESBO

Item Type:	Article (Journal)
Additional Information:	7122/43217
Uncontrolled Keywords:	Epoxidized soybean oil; foods stored in glass jars with metal lids; gas chromatography-mass spectrometry
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Chemistry
Depositing User:	Dr MAZIDATULAKMAM MISKAM
Date Deposited:	09 Jun 2015 10:11
Last Modified:	04 Jan 2018 16:38
URI:	http://irep.iium.edu.my/id/eprint/43217

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