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Alpha-mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells via PPAR gamma, GLUT4, and leptin expressions

Bakhtiar, M. Taher and Mohamed Amiroudine, Mohamed Zaffar Ali and Tengku Zakaria, Tengku Muhamad Faris Syafiq and Darnis, Deny Susanti and Arief Ichwan, Solachuddin Jauhari and Kaderi, Mohd Arifin and Ahmed, Qamar Uddin and Zakaria, Zainul Amiruddin (2015) Alpha-mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells via PPAR gamma, GLUT4, and leptin expressions. Evidence-Based Complementary and Alternative Medicine (740238). pp. 1-9. ISSN 1741-427X

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Abstract

Obesity has been often associated with the occurrence of cardiovascular diseases, type 2 diabetes, and cancer. The development of obesity is also accompanied by significant differentiation of preadipocytes into adipocytes. In this study, we investigated the activity of alpha-mangostin, a major xanthone component isolated from the stem bark of G. malaccensis, on glucose uptake and adipocyte differentiation of 3T3-L1 cells focusing on PPAR gamma, GLUT4, and leptin expressions. alpha-Mangostin was found to inhibit cytoplasmic lipid accumulation and adipogenic differentiation. Cells treated with 50 mu M of alpha-mangostin reduced intracellular fat accumulation dose-dependently up to 44.4% relative to MDI-treated cells. Analyses of 2-deoxy-D-[H-3] glucose uptake activity showed that alpha-mangostin significantly improved the glucose uptake (P < 0.05) with highest activity found at 25 mu M. In addition, alpha-mangostin increased the amount of free fatty acids (FFA) released. The highest glycerol release level was observed at 50 mu M of alpha-mangostin. qRT-PCR analysis showed reduced lipid accumulation via inhibition of PPAR gamma gene expression. Induction of glucose uptake and free fatty acid release by alpha-mangostin were accompanied by increasing mRNA expression of GLUT4 and leptin. These evidences propose that alpha-mangostin might be possible candidate for the effective management of obesity in future.

Item Type: Article (Journal)
Additional Information: 5209/42492
Uncontrolled Keywords: lipid droplet accumulation, garcinia-mangostana, signaling pathway, down-regulation, adipose-tissue, tumor-growth, obesity, adipogenesis, xanthones, mechanism
Subjects: R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User: Dr Muhammad Taher
Date Deposited: 21 Apr 2015 16:18
Last Modified: 25 Sep 2017 15:23
URI: http://irep.iium.edu.my/id/eprint/42492

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