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Simple process capability analysis and quality validation of monoclonal antibody production in bench-top bioreactor

Mel, Maizirwan and Arifin, Mohd Azmir and Abdul Karim, Mohamed Ismail and Yusof, Faridah (2011) Simple process capability analysis and quality validation of monoclonal antibody production in bench-top bioreactor. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, The Legend Hotel, Kuala Lumpur.

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Abstract

The production of monoclonal antibody (IgM) for anti-cancer by fermentation of hybridoma cell in benchtop bioreactor has been investigated. The main target of this study was to increase the percentage of cell viability and to achieve high yield of IgM. There are many ways that can be applied to improve the process and one of them is by choosing the correct Six Sigma’s Design of Experiment (DOE). In this study, Taguchi’s Experimental Design has been applied to achieve high percentage of cell viability in the fermentation experiment. The process capability of this study was later analyzed by using the MiniTab Software (Ver. 14). Analysis has shown that this study has fulfilled a minimum target of process improvement as indicated by the Cp and Cpk value of 0.90 and 0.86. This proved that the fermentation experiment has achieved cell viability of more than 80% throughout the study.

Item Type: Conference or Workshop Item (Full Paper)
Additional Information: 4085/4232
Uncontrolled Keywords: Monoclonal antibody, hybridoma, process capability, aguchi experimental design
Subjects: T Technology > TP Chemical technology > TP155 Chemical engineering
T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr Maizirwan Mel
Date Deposited: 05 Oct 2011 12:30
Last Modified: 05 Oct 2011 14:42
URI: http://irep.iium.edu.my/id/eprint/4232

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