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Study of cells attachment using impedance spectroscopy technique

Arifuzzaman, S. M. and Nordin, Anis Nurashikin and Mel, Maizirwan and Spray, David and Voiculescu, Ioana (2011) Study of cells attachment using impedance spectroscopy technique. In: ASME International Mechanical Engineering Congress and Exposition (ASME 2011), 11-17 November 2011, Denver Colorado. (In Press)

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Abstract

In this paper we report on the electrical impedance spectroscopy characterization of the attachment of two mammalian cell lines: endothelial cells (RFPEC) and fibroblast cells (V79). For electrical cell–substrate impedance sensing (ECIS) of the endothelial cells a commercially available eightwell cell culture impedance array (ECIS-8W1E) was used. The impedance measurements were recorded with cell culture medium (without cells) and with endothelial cells or fibroblast cells in the culture medium over a frequency range from 100 Hz to 100 kHz. The impedance measurements were compared to the equivalent circuit model. The impedance measurements were also simulated using COMSOL MultiphysicsTM, a commercially available modeling package.

Item Type: Conference or Workshop Item (Full Paper)
Additional Information: 3239/4226
Uncontrolled Keywords: cell–substrate impedance sensing (ECIS), impedance, reactance, eight-well cell culture impedance array, COMSOL Multiphysic
Subjects: T Technology > T Technology (General) > T175 Industrial research. Research and development
T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Engineering
Kulliyyah of Engineering > Department of Biotechnology Engineering
Kulliyyah of Engineering > Department of Electrical and Computer Engineering
Depositing User: Dr Maizirwan Mel
Date Deposited: 12 Sep 2011 17:10
Last Modified: 12 Sep 2011 17:10
URI: http://irep.iium.edu.my/id/eprint/4226

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