Comparison of alcohol free GSH production by ultrasonic and homogenizing method

Mohamed Salleh, Mohamad Ramlan and Jamal, Parveen and Gad, Abd. Elaziem Farouk and Hashim, Yumi Zuhanis Has-Yun and Salleh, Rohani and Hasim, Haslinda (2008) Comparison of alcohol free GSH production by ultrasonic and homogenizing method. Iranian Journal of Chemistry & Chemical Engineering, 27 (4). pp. 107-113. ISSN 1021-9986

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Abstract

Homogenizer and ultrasonicator that we call as mechanical methods were used in the lab scale production of alcohol free reduced glutathione (alcohol free GSH) from baker’s yeast. The study was done to determine the effects of specific parameters of the methods and the pH of the medium on the production of the GSH. The cell disruptions were run at a wide range of those parameters at the suitable common parameters of the methods. The disrupted cells suspension was then centrifuged at 12,000 rpm for 20 min and the GSH content in the supernatant was analyzed by taking the absorbance (A) value of the solution by spectrophotometer at 412 nm. The results obtained showed that the production of GSH was affected by the adjusted specific parameters of each method and the yield of production by the homogenizing and ultrasonic method was found 3.3 and 4.1 times, respectively, better than the yield of production by the unadjusted parameters. Ultrasonic method which produced 23.2 mg/L product was found 1.3 times better than the homogenizing method (18.5 mg/L) in producing the GSH. The result obtained from the lab scale study of the above mentioned mechanical methods showed that the implementation of them in the large scale production of the alcohol free GSH is possible.

Item Type:	Article (Journal)
Additional Information:	2937/4108
Uncontrolled Keywords:	Alcohol free GSH; Baker's yeast; Cells disruption; Homogenizing method; Specific parameters; Ultrasonic method
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Prof.Dr. Parveen Jamal
Date Deposited:	19 Jul 2013 11:00
Last Modified:	19 Jul 2013 11:00
URI:	http://irep.iium.edu.my/id/eprint/4108

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