Comparison studies of GSH isolation from several Malaysian local fruits by mechanical methods

Baiti, Arif Afraha and Jamal , Parveen and Mirghani, Mohamed Elwathig Saeed (2010) Comparison studies of GSH isolation from several Malaysian local fruits by mechanical methods. Research Journal of Chemistry and Environment, Spcial. pp. 299-302. ISSN 0972-0626

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Abstract

Glutathione (GSH) is a simple tripeptide produced by the liver. It is a volatile substance, which is sweet in taste, comprised of the amino acids cysteine, glutamic acid and glycine. In these modern days, the use of traditional medicine by the suffers of chronic disease is encouraged due to the adverse effects of chemical drugs and treatment using medicines of natural origin appears to offer more gentle means of managing such disease. Therefore, the practice of traditionally used natural plants as medicines is one of the alternative ways to cure the illnesses. Besides, this study was done in order to investigate the production of GSH from fruits since most of the studies that had been conducted before were done on the isolation of GSH from other sources. In this study the reduced glutathione (GSH) was isolated from a potential local fruit of Malaysia. Three mechanical methods; homogenizer, ultrasonic and autolysis were screened for disrupting the fruits cells for maximum GSH content. Different methods of cell disruption gave different effect on the GSH production. Watermelon (Citrullus lanatus) is the best potential local fruit that contain higher GSH followed by Jackfruit (Artocarpus heterophylus) and Sapodilla (Manilkara zapota), while ultrasonic is the best cell disruption method which can give the maximum amount of GSH, followed by homogenizer method and autolysis method.

Item Type:	Article (Journal)
Additional Information:	2937/39
Uncontrolled Keywords:	autolysis, GSH, homogenizer, Malaysian fruits, ultrasonic
Subjects:	R Medicine > R Medicine (General) T Technology > TP Chemical technology T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Prof.Dr. Parveen Jamal
Date Deposited:	21 Sep 2011 16:54
Last Modified:	24 Jan 2012 17:53
URI:	http://irep.iium.edu.my/id/eprint/3937

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