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Dynamics behavior of Endoglucanase from Fusarium oxysporum

Noorbatcha, Ibrahim Ali and S., Waesoho and Mohd. Salleh, Hamzah (2011) Dynamics behavior of Endoglucanase from Fusarium oxysporum. In: International Conference on Biotechnology Engineering, ICBioE’11, 17-19 May, 2011 , Kuala Lumpur.

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Molecular dynamics methods are very useful tool in understanding the behavior of the enzymes at higher temperatures. It is well known that some enzymes lose their enzyme activity at higher temperatures. In this work we employ molecular dynamics simulation of an endoglucanase from Fusarium oxysporum (EGFO) to understand the reasons behind the loss of enzyme activity when the temperatures is increased form 40°C to 80°C. The dynamic behavior of this enzyme is analyzed by examining the Root Mean Square Derivation(RMSD), salt bridges, gyration ratio and hydrogen bonds at different temperatures as a function of time. It has been found that there are differences in (i) the RMSD of backbone and coil regions, (ii) the number of hydrogen bonds among amino acidresidues and between amino acid residues and explicit watermolecules within the coil region of the enzyme (iii) total number of salt bridges, and (iv) the gyration ratio of the enzyme at different temperatures. These factors could play significant role in the loss of activity of EGFO as the temperature is increased.

Item Type: Conference or Workshop Item (Full Paper)
Additional Information: 3704/3911 ISBN:978-983-42978-3-1
Uncontrolled Keywords: Endoglucanase; Molecular dynamics simulation; coil regions; Fusarium oxysporum; thermostability
Subjects: T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Prof. Dr. Ibrahim Ali Noorbatcha
Date Deposited: 17 Oct 2011 12:10
Last Modified: 16 Nov 2011 10:19
URI: http://irep.iium.edu.my/id/eprint/3911

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