Immobilization of lipase enzyme by low cost material: a statistical approach

Alam, Md Zahangir and Asih, Devi Ratna and Salleh, Md. Noor (2014) Immobilization of lipase enzyme by low cost material: a statistical approach. International Journal of Biological, Veterinary, Agricultural and Food Engineering, 8 (6). pp. 522-525.

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Abstract

Immobilization of lipase enzyme produced from palm oil mill effluent (POME) by the activated carbon (A C) among the low cost support materials was optimized. The results indicated that immobilization of 94% was achieved by AC as the most suitable support material. A sequential optimization strategy based on a statistical experimental design, including one-fact or-at-a-time (OFAT) method was used to determine the equilibrium time. Three components influencing lipase immobilization were optimized by theresponse surface methodology (RSM) based on the face-centered central composite design (FCCCD). On the statistical analysis of the results, the optimum enzyme concentration loading, agitation rate and carbon active dosage were found to be 30 U/ml, 300 rpm and 8 g/L respectively, with a maximum immobilization activity of 3732.9 U/g-AC after 2 hrs of immobilization. Analysis of variance (ANOVA) showed a high regression coefficient (R2) of 0.999, which indicated a satisfactory fit of the model with the experimental data. The parameters were statistically significant at p<0.05.

Item Type:	Article (Journal)
Additional Information:	4157/38274
Uncontrolled Keywords:	Activated carbon, adsorption, immobilization, POME based lipase
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr. Md. Zahangir Alam
Date Deposited:	15 Sep 2014 14:59
Last Modified:	12 Jun 2018 15:44
URI:	http://irep.iium.edu.my/id/eprint/38274

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