Pilot-scale production of lipase using palm oil mill effluent as a basal medium and its immobilization by selected materials

Asih, Devi Ratna and Alam, Md Zahangir and Salleh, Md. Noor and Salihu, Aliyu (2014) Pilot-scale production of lipase using palm oil mill effluent as a basal medium and its immobilization by selected materials. Journal of Oleo Science, 63 (8). pp. 779-785. ISSN 1345-8957 E-ISSN 1347-3352

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Abstract

A pilot-scale production of lipase using palm oil mill effluent (POME) as a fermentation basal medium was carried out, and parameters for immobilization of the produced lipase were optimized. Lipase production in a 300-L bioreactor was performed using two proposed strategies, constant power per volume (P/V) and constant tip speed. Moreover, lipase immobilization on different materials was also investigated. Lipase production was performed using liquid-state bioconversion of POME as the medium and Candida cylindracea as the inoculum. The fermentation medium was composed of 1% total suspended solids (TSS) of POME, 0.5% (w/v) peptone, 0.7% (v/v) Tween-80, and 2.2% inoculum. The medium composition was decided on the basis of the medium optimization results of a previous study. The fermentation was carried out for 48 h at 30°C and pH 6. The maximum lipase production was 5.72U/mL and 21.34 U/mL, obtained from the scale-up strategies of constant tip speed and P/V, respectively. Four accessible support materials were screened for their potential use in immobilization. The most suitable support material was found to be activated carbon, with a maximum immobilization of 94%.

Item Type:	Article (Journal)
Additional Information:	4157/38191
Uncontrolled Keywords:	Candida cylindraceae; Immobilization; Lipase; Scale up strategy; Stirred tank bioreactor (300L)
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr. Md. Zahangir Alam
Date Deposited:	12 Sep 2014 09:18
Last Modified:	10 Aug 2017 15:21
URI:	http://irep.iium.edu.my/id/eprint/38191

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