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Effects of NAA and BAP, double-layered media, and light distance on in vitro regeneration of nelumbo nucifera Gaertn. (Lotus), an aquatic edible plant

Mahmad, Noraini and Mat Taha, Rosna and Othman, Rashidi and Saleh, Azani and Hasbullah, Nor Azlina and Elias, Hashimah (2014) Effects of NAA and BAP, double-layered media, and light distance on in vitro regeneration of nelumbo nucifera Gaertn. (Lotus), an aquatic edible plant. The Scientific World Journal, 2014 (745148). pp. 1-8. ISSN 1537-744X

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Abstract

In vitro direct regeneration of Nelumbo nucifera Gaertn. was successfully achieved from immature explants (yellow plumule) cultured on a solid MS media supplemented with combinations of 0.5 mg/L BAP and 1.5 mg/L NAA which resulted in 16.00 ± 0.30 number of shoots per explant and exhibited a new characteristic of layered multiple shoots, while normal roots formed on the solid MS basal media. The double-layered media gave the highest number of shoots per explant with a ratio of 2 : 1 (liquid to solid) with a mean number of 16.67 ± 0.23 shoots per explant with the formation of primary and secondary roots from immature explants. In the study involving light distance, the tallest shoot (16.67 ± 0.23 mm) obtained from the immature explants was at a light distance of 200 mm from the source of inflorescent light (1000 lux). The plantlets were successfully acclimatized in clay loam soil after 8 months being maintained under in vitro conditions.

Item Type: Article (Journal)
Additional Information: 3842/37971
Uncontrolled Keywords: acclimatization; article; clay; controlled study; explant; in vitro study; inflorescence; light; liquid; loam soil; Murashige and Skoog medium; Nelumbo nucifera; nonhuman; plant regeneration; plant root; plant tissue; shoot; solid
Subjects: S Agriculture > S Agriculture (General)
S Agriculture > SB Plant culture
T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Architecture and Environmental Design > Department of Landscape Architecture
Depositing User: Dr rashidi othman
Date Deposited: 03 Sep 2014 10:14
Last Modified: 19 Sep 2017 10:21
URI: http://irep.iium.edu.my/id/eprint/37971

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