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Identification of putative Cof-like hydrolase associated with dehalogenase in Enterobacter cloacae MN1 isolated from the contaminated sea-side area of the Philippines

Abdul Hamid, Azzmer Azzar and Nemati, Mahdieh and Abdulghader, Mohamed Faraj and Gicana, Ronie G and Ibtrahim, Nurhafizah and Huyop, Fahrul Zaman (2013) Identification of putative Cof-like hydrolase associated with dehalogenase in Enterobacter cloacae MN1 isolated from the contaminated sea-side area of the Philippines. Malaysian Journal of Microbiology, 9 (3). pp. 253-259. ISSN 1823-8262

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Abstract

Aims: The present study aimed at molecular identification of putative Cof-like hydrolase associated with dehalogenase gene from a bacterium that was isolated from a contaminated sea-side area in the Philippines. The bacterium was subjected to 16S rRNA gene sequence analysis for identity of the genus and species. Methodology and results: Based on basic microbiological analysis and 16S rRNA sequence determination, strain MN1 showed high sequence identity to Enterobacter cloacae. This is the first reported study that Enterobacter could degrade 2,2-dichloropropionate (2,2-DCP). A putative dehalogenase gene like was identified by direct sequencing and analysis of the PCR-amplified genomic DNA of the bacterium. A comparative analysis of the sequence data revealed that the amino acid sequence is closely related to several Cof-like hydrolase associated with L-specific dehalogenases. Conclusion, significance and impact of study: Current study may suggest that the hydrolase may have similar function to dehalogenase. However, further analysis like enzyme assay need to be carried out to confirm this. Putative dehalogenase gene can be amplified using PCR technique provided that the specific primers designed were used.

Item Type: Article (Journal)
Additional Information: 7089/37515
Uncontrolled Keywords: Enterobacter cloacae MN1, pollutant degradation, Cof-like hydrolase, dehalogenase
Subjects: T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Science > Department of Biotechnology
Depositing User: Br Azzmer Azzar Abdul Hamid
Date Deposited: 13 Aug 2014 10:07
Last Modified: 13 Aug 2014 10:07
URI: http://irep.iium.edu.my/id/eprint/37515

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