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Optimization of lipase production by Candida cylindracea in palm oil mill effluent based medium using statistical experimental design

Salihu, Aliyu and Alam, Md Zahangir and AbdulKarim, M. Ismail and Mohd. Salleh, Hamzah (2011) Optimization of lipase production by Candida cylindracea in palm oil mill effluent based medium using statistical experimental design. Journal of Molecular Catalysis B: Enzymatic, 69 (1-2). pp. 66-73. ISSN 1381-1177

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Abstract

Sequential optimization strategy based on statistical experimental design including one-factor-at-a-time (OFAT) method was used to enhance the production of lipase by Candida cylindracea ATCC 14830 using palm oil mill effluent as a basal medium in shake flask cultures. The two-level Plackett–Burman (PB) design was implemented to screen the medium components that significantly influence the production. Following the OFAT method, three significant components influencing lipase production were identified as peptone, Tween-80 and inoculum. The optimum values of these three components were determined by response surface methodology (RSM) based on the face-centered central composite design (FCCCD). The validity of the model developed was verified, and the optimum medium containing 0.45% (w/v) peptone, 0.65% (v/v) Tween-80 and 2.2% (v/v) inoculum led to amaximumlipase production of 20.26 U/ml, which was 5.19-fold higher than the unoptimized medium. The analysis of variance indicated that the established model was significant (P < 0.05). This study innovatively developed a fermentation medium utilizing renewable palm oil mill effluent (POME) to produce lipase at considerable level.

Item Type: Article (Journal)
Additional Information: 4157/3739
Uncontrolled Keywords: Candida cylindracea; Lipase production; Optimization; Face-centered central composite design; Palm oil mill effluent
Subjects: Q Science > QD Chemistry
T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr Hamzah Mohd. Salleh
Date Deposited: 21 Sep 2011 16:59
Last Modified: 10 Aug 2017 10:59
URI: http://irep.iium.edu.my/id/eprint/3739

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