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Effect of process parameters on lipase production by Candida cylindracea in stirred tank bioreactor using renewable palm oil mill effluent based medium

Salihu, Aliyu and Alam, Md Zahangir and AbdulKarim, M. Ismail and Mohd. Salleh, Hamzah (2011) Effect of process parameters on lipase production by Candida cylindracea in stirred tank bioreactor using renewable palm oil mill effluent based medium. Journal of Molecular Catalysis B: Enzymatic, 72 ( ). pp. 187-192. ISSN 1381-1177

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Abstract

A two-level full factorial design (FFD) was employed to determine the effects of process parameters on lipase production by Candida cylindracea ATCC 14830 in palm oil mill effluent (POME)-based medium. Ten experimental runs based on three parameters (temperature, agitation and aeration) as indicated by the FFD were carried out in a stirred-tank bioreactor. On statistical analysis of the results, the optimum temperature, aeration and agitation rates were found to be 30 ◦C, 1.0 vvm and 400 rpm respectively, with a maximum activity of 41.46 U/ml after 36 h of fermentation. Analysis of variance (ANOVA) showed a high coefficient of determination (R2) value of 0.999, indicating a satisfactory fit of the model with the experimental data. All the three parameters were statistically significant at p < 0.05. The validation experiment also confirmed that apart from lipase production, there was an increase in chemical oxygen demand (COD) removal throughout the fermentation period.

Item Type: Article (Journal)
Additional Information: 4157/3736
Uncontrolled Keywords: Lipase, Palm oil mill effluent, Stirred-tank bioreactor, Fermentation, Full factorial design, Optimization
Subjects: Q Science > QD Chemistry
T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr Hamzah Mohd. Salleh
Date Deposited: 21 Sep 2011 14:48
Last Modified: 10 Aug 2017 10:44
URI: http://irep.iium.edu.my/id/eprint/3736

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