IIUM Repository

The growth study of vero cells in different type of microcarrier

Ahmad Nor, Yusilawati and Sulong, Nurul Hafizah and Mel, Maizirwan and Mohd. Salleh, Hamzah and Sopyan, Iis (2010) The growth study of vero cells in different type of microcarrier. Materials Sciences and Applications, 1 (5). pp. 261-266. ISSN 2153-1188 (O), 2153-117X (P)

[img] PDF (the growth study of vero) - Published Version
Restricted to Registered users only

Download (2MB) | Request a copy

Abstract

The fact of microcarrier (MC) culture introduces new possibilities and makes possible the practical high-yield culture of anchorage-dependent cells has generated a considerable focus in this study. The objective of this research was to study the comparison of Vero cell growth on different types of commercial microcarriers; Cytodex-1, Cytodex-3, Hillex® II and Plastic Plus in spinner vessel and two liters bioreactor cultured for 96 hours. Biological performance of the microcarrier in RPMI media showed the preference of Vero cell grew on Cytodex 3 microcarriers with highest maximum viable cell number (2.4 × 105 cells/ml) followed by Cytodex 1, Hillex and Plustic Plus. Vero cell on Cytodex- 3 data in spinner flask was compared in bioreactor and result showed higher viable cell number in biorector. Thus, this dextran-crosslink gelatin microcarrier (Cytodex 3) provided the best surface for cell attachment and fast proliferation. At the end of this cell growth improvement will be used for virus transfection producing a vaccine in bioreactor.

Item Type: Article (Journal)
Additional Information: 4085/3721
Uncontrolled Keywords: cytodex 3, microcarrier, spinner flask, vero cells, vaccines
Subjects: T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr Hamzah Mohd. Salleh
Date Deposited: 22 Sep 2011 08:54
Last Modified: 06 Jul 2021 09:43
URI: http://irep.iium.edu.my/id/eprint/3721

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year