Water toxicity detection using cell-based hybrid biosensors

Fei, Liu and Voiculescu, Ioana and Nordin, Anis Nurashikin and Fang, Li (2013) Water toxicity detection using cell-based hybrid biosensors. In: 2013 IEEE Sensors, 3-6 Nov. 2013, Baltimore, MD, USA.

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Abstract

This paper presents a novel cell-based biosensor device that contains a quartz crystal microbalance (QCM), integrated with the electric cell-substrate impedance sensing(ECIS) technique. Bovine aortic endothelial cells (BAECs) are cultivated on the sensors and monitored in parallel by both sensors. When two different sensors monitors the same BAECs monolayer cultures on these sensors the information provided by these sensors is more accurate. Cell culturing microwells were fabricated and glued on this biosensor device for minimizing media and reagent demands and potential automation. The microwells were designed in a manner that can eliminate the fluid shear stress and allow laminar flow for the media and other fluids introduced in the cell-culturing chamber during testing. We demonstrated that BAECS cells could be cultured in these microwells. Resonant frequency and impedance spectroscopy measurements were performed during a time interval. It was demonstrated that this cell- based sensor could be used for toxicity experiments.

Item Type:	Conference or Workshop Item (Full Paper)
Additional Information:	3239/36876 (DOI: 10.1109/ICSENS.2013.6688374)
Uncontrolled Keywords:	water toxicity detection
Subjects:	T Technology > TK Electrical engineering. Electronics Nuclear engineering > TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Electrical and Computer Engineering
Depositing User:	Dr. Anis Nurashikin Nordin
Date Deposited:	09 Jun 2014 17:01
Last Modified:	09 Jun 2014 17:01
URI:	http://irep.iium.edu.my/id/eprint/36876

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