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Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design

Jamal, Parveen and Azmi, Saiful Mohammad Nizam and Amid, Azura and Mohd. Salleh, Hamzah and Hashim, Yumi Zuhanis Has-Yun (2014) Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design. Advances in Environmental Biology, 8 (3). pp. 734-743. ISSN 1998-1066 (O), 1995-0756 (P)

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Abstract

Xanthine oxidase (XO) is a key enzyme in hyperuricemia, catalyzing the oxidation of hypoxanthine to xanthine and then to uric acid. Excess serum accumulated with uric acid leads to a type of arthritis known as gout. In this study, development of process conditions for XO inhibitory activity from the leaves of Morinda elliptica was performed by using 70% methanol. Optimization of process parameters such as extraction temperature (ºC), extraction time (h), agitation speed (rpm) and ratio of sample to solvent (1g/ml) at five levels was carried out using central composite design (CCD) for the improvement of activity to treat gout. The analysis of variance demonstrated that the model F-value of 18.31 showed the significance of the model with R2 of 97.71%. The analysis revealed that the percentage of XO inhibitory activity was improved at 32 ºC, 30 h, 125 rpm and 1 g/15 ml of solvent. The optimized conditions were verified and the percentage of XO inhibitory activity obtained was 88.93%. The results are encouraging to formulate food, nutraceutical or pharmaceutical products incorporating natural xanthine oxidase inhibitor (XOI), an alternative to irresponsive synthetic XOI.

Item Type: Article (Journal)
Additional Information: 2937/36413
Uncontrolled Keywords: Morinda elliptica, Xanthine oxidase inhibitor, Gout, Optimisation, Central composite design.
Subjects: T Technology > T Technology (General)
T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering
Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Prof.Dr. Parveen Jamal
Date Deposited: 23 Jul 2014 11:55
Last Modified: 19 Sep 2017 12:30
URI: http://irep.iium.edu.my/id/eprint/36413

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