Comparing BRIN-BD11 culture producing insulin using different type of microcarriers

Mel, Maizirwan and Abdul Karim, Mohamed Ismail and Mohd Yusof, Siti Aishah and Hashim, Yumi Zuhanis Has-Yun and Ahmad Nor, Yusilawati (2010) Comparing BRIN-BD11 culture producing insulin using different type of microcarriers. Cytotechnology, 62. pp. 423-430. ISSN 1573-0778 (O), 0920-9069 (P)

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Abstract

This research was conducted to examine the growth profile, growth kinetics, and insulinsecretory responsiveness of BRIN-BD11 cells grown in optimized medium on different types of microcarriers (MCs). Comparisons were made on modified polystyrene (Hillex� II) and crosslinked polystyrene Plastic Plus (PP) from Solohill Engineering. The cell line producing insulin was cultured in a 25 cm2 T-flask as control while MCs based culture was implemented in a stirred tank bioreactor with 1 L working volume. For each culture type, the viable cell number, glucose, lactate, glutamate, and insulin concentrations were measured and compared. Maximum viable cell number was obtained at 1.47 9 105 cell/mL for PP microcarrier (PPMCs) culture, 1.35 9 105 cell/mL Hillex� II (HIIMCs) culture and 0.95 9 105 cell/mL for T-flask culture, respectively. The highest insulin concentration has been produced in PPMCs culture (5.31 mg/L) compared toHIIMCs culture (2.01 mg/L) and T-flask culture (1.99 mg/L). Therefore overall observation suggested that PPMCs was likely preferred to be used for BRIN-BD11 cell culture as compared with Hillex� II MCs.

Item Type:	Article (Journal)
Additional Information:	4085/3639
Uncontrolled Keywords:	insulin, BRIN-BD11, solohill, microcarrier, bioreactor
Subjects:	T Technology > TP Chemical technology > TP155 Chemical engineering T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr Maizirwan Mel
Date Deposited:	22 Sep 2011 11:54
Last Modified:	22 Sep 2011 11:54
URI:	http://irep.iium.edu.my/id/eprint/3639

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