Transition state analysis of Pyranose 2-Oxidase from Peniophora sp.

Ibrahim Ali , Noorbatcha and Ramli, Siti Khairani and Salleh, Hamzah Mohd. (2013) Transition state analysis of Pyranose 2-Oxidase from Peniophora sp. In: International Conference on Biotechnology Engineering (ICBioE2013), 2-4 July 2013, Kuala Lumpur, Malaysia.

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Abstract

Significant interest has been aroused for the use of pyranose 2-oxidase (P2Ox) as a catalyst in biotechnological applications particularly in biofuel and food technology. Understanding the transition state of this enzyme offers greater possibility to improve the catalytic activity of this enzyme and thus speeding the industrial processes. The main purpose of this study is to identify and characterize the transition states for the catalytic reaction of P2Ox from white-rot fungus Peniophora sp.. The complete path for the oxidation reaction catalyzed by P2Ox was investigated using the PM7 semi-empirical method with MOZYME function as implemented in the MOPAC 2012 (Molecular Orbital PACkage) program. The results of this study was consistent with the experimental method where appreciable conformational changes can be seen in the loop of residues 454-461 which was anticipated to be functions as a gatekeeper to allow the substrate to pass into the active center. Keywords: pyranose-2-oxidase, fungus, transition state, PM7, MOZYME

Item Type:	Conference or Workshop Item (Full Paper)
Additional Information:	3704/34141 (ISBN: ISBN: 978-983-42978-6-2)
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Prof. Dr. Ibrahim Ali Noorbatcha
Date Deposited:	13 Jan 2014 16:25
Last Modified:	15 Jan 2014 10:54
URI:	http://irep.iium.edu.my/id/eprint/34141

Available Versions of this Item

• Transition state analysis of Pyranose 2-Oxidase from Peniophora sp. (deposited 13 Jan 2014 16:25) [Currently Displayed]
  • Molecular docking of Cellobiose and Cellotetraose into an Endoglucanase from Fusarium oxysporum. (deposited 15 Jan 2014 10:54)

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