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Cloning and expression of Voltage-dependent Anion Channel 2 (VDAC2) from electrically stimulated chicken

Ahmad Hassan, Nurhidayah and Amid, Azura and Mohd. Salleh, Hamzah (2013) Cloning and expression of Voltage-dependent Anion Channel 2 (VDAC2) from electrically stimulated chicken. Journal of Pure and Applied Microbiology, 7 (Spec.). pp. 43-52. ISSN 0973-7510

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Abstract

VDAC2 protein identified to be overexpressed in stunned chicken is a potential biomarker in differentiating stunned chicken since the expression was proportional to the voltage applied. To facilitate research, the gene encoding VDAC is cloned, expressed and optimized in BL21-AI Escherichia coli. cDNA was synthesized from mRNA of stunned chicken’s skeletal muscle and cloned using recombination reaction. All positive clones were identified by PCR and restriction enzyme digestion as well as DNA sequencing. Recombinant VDAC2 protein was purified by Ni-NTA spin-column. Western blot performed using polyclonal anti-N terminal human VDAC2 and anti-His tag revealed a 34 kDa protein, confirming the expression of recombinant VDAC2.

Item Type: Article (Journal)
Additional Information: 3688/34096
Uncontrolled Keywords: Gallus gallus, electrical stimulation, Escherichia coli, recombinase reaction
Subjects: T Technology > T Technology (General) > T175 Industrial research. Research and development
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Prof Dr Azura Amid
Date Deposited: 13 Jan 2014 13:51
Last Modified: 16 Mar 2015 09:01
URI: http://irep.iium.edu.my/id/eprint/34096

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