Development of new media formulation for the improvement of recombinant protein expression in E.Coli

Mohd. Salleh, Hamzah and Hashim, Yumi Zuhanis Has-Yun and Nuge, Tamrin (2012) Development of new media formulation for the improvement of recombinant protein expression in E.Coli. Research Report. s.n, Kuala Lumpur. (Unpublished)

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Abstract

The optimum growth media for the recombinant protein expression has the added advantage of improved cost effectiveness, enhanced down-stream processing, reduced wastewater, lower production costs and reduced investment in equipment. In this study a series of statistical optimization involves screening, classical optimization (OFAT) and response surface methodology (RSM) is employed to develop an optimum media for bacteria growth. E. coli DH5 harboring phytase gene from Mycobacterium smegmatis is used as a model to develop the modified growth media. At the end of the study, the effectiveness of the newly developed growth media was also tested on few recombinant proteins.

Item Type:	Monograph (Research Report)
Additional Information:	1813/33684
Uncontrolled Keywords:	phytase, E. coli, growth media, optimization, recombinant protein
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Sr. Norsyaziela Zulkefli
Date Deposited:	13 May 2015 14:35
Last Modified:	13 May 2015 14:35
URI:	http://irep.iium.edu.my/id/eprint/33684

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