In-vitro antibacterial activity of crude defatted Moringa Oleifera seed extract: kill time study

Idris, Munirat A. and Muyibi, Suleyman Aremu and Abdulkarim, Mohd Ismail and Jamal, Parveen and Jami, Mohammed Saedi (2013) In-vitro antibacterial activity of crude defatted Moringa Oleifera seed extract: kill time study. Australian Journal of Basic and Applied Sciences, 7 (6). pp. 149-153. ISSN 1991-8178

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Abstract

Earlier research conducted on moringa oleifera seed extract not only reveals its excellent coagulant property; it also being proven to possesses antibacterial activity against microorganism. An antibacterial agent 4(α-L- rhamnosyloxy) benzyl isothiocyanate, a plant synthesized derivatives of benzyl isothiocyanates has been identified as the active antibacterial agent present in the seed. In this study, the mode of antibacterial action was determined using the kill-time study on e.coil and pseudosomonas aeruginosa bacterial strains. The minimum inhibitory concentration (MIC) assay was done using macrodilution broth. MIC values were varied from 0.0125mg/ml to 0.1mg/ml. Kill-time assay was carried out based on standard procedure. The growth of the microbes was monitored for every 30 minutes by viability counting on agar plate. Log reduction of viable cells counts ranged from 0 to 3.6 log10 for e.coli and 1log10 to 4log10 for p.aeruginosa. The results of the kill-time study revealed that the extract was bactericidal against e.coli and p.aeruginosa within 30 minutes of contact resulting in about 90% elimination of the strains. Most of the microbes were killed at high MIC value of 0.1 mg/ml, which might suggest that the seed extract exhibits concentration-dependent killing that revealed that it can be used as a natural disinfectant for water treatment. Key words: benzyl isothiocyanates; Moringa oleifera; kill-time; e.coli; p. aeruginosa

Item Type:	Article (Journal)
Additional Information:	4164/32221
Uncontrolled Keywords:	benzyl isothiocyanates; Moringa oleifera; kill-time; e.coli; p. aeruginosa
Subjects:	T Technology > TD Environmental technology. Sanitary engineering > TD201 Water supply for domestic and industrial purposes
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Prof SULEYMAN AREMU MUYIBI
Date Deposited:	08 Oct 2013 09:08
Last Modified:	21 Jul 2014 10:00
URI:	http://irep.iium.edu.my/id/eprint/32221

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