Hepatoprotective activity of methanolic extract of bauhinia purpurea leaves against paracetamol-induced hepatic damage in rats

Yahya, Farhana and Mamat, Siti Syariah and Kamarolzaman, Mohammad Fauzi Fahmi and Seyedan, Atefeh Alsadat and Jakius, Krystal Fredoline and Mahmood, Nur Diyana and Shahril, Mohamad Syahmi and Suhaili, Z and Mohtarrudin, Nurhafiza and Darnis, Deny Susanti and Somchit, MN and Teh, L. K. and Salleh, M. Z. and Zakaria, Zainul Amiruddin (2013) Hepatoprotective activity of methanolic extract of bauhinia purpurea leaves against paracetamol-induced hepatic damage in rats. Evidence-Based Complementary and Alternative Medicine, 2013. ISSN 1741-427X

PDF (Hepatoprotective activity of methanolic extract of bauhinia purpurea leaves against paracetamol-induced hepatic damage in rats) - Published Version Restricted to Repository staff only Download (3MB) | Request a copy

Abstract

In an attempt to further establish the pharmacological properties of Bauhinia purpurea (Fabaceae), hepatoprotective potential of methanol extract of B. purpurea leaves (MEBP) was investigated using the paracetamol- (PCM-) induced liver toxicity in rats. Five groups of rats (n = 6) were used and administered orally once daily with 10% DMSO (negative control), 200 mg/kg silymarin (positive control), or MEBP (50, 250, and 500 mg/kg) for 7 days, followed by the hepatotoxicity induction using paracetamol (PCM). The blood samples and livers were collected and subjected to biochemical and microscopical analysis. The extract was also subjected to antioxidant study using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay with the total phenolic content (TPC) also determined. From the histological observation, lymphocyte infiltration and marked necrosis were observed in PCM-treated groups (negative control), whereas maintenance of the normal hepatic structural was observed in group pretreated with silymarin and MEBP. Hepatotoxic rats pretreated with silymarin or MEBP exhibited significant decrease (P < 0.05) in ALT and AST enzyme level. Moreover, the extract also exhibited antioxidant activity and contained high TPC. In conclusion, MEBP exerts potential hepatoprotective activity that could be partly attributed to its antioxidant activity and high phenolic content and thus warrants further investigation.

Item Type:	Article (Journal)
Additional Information:	5641/30576
Subjects:	R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science
Depositing User:	Dr Deny Susanti Darnis
Date Deposited:	05 Aug 2013 14:29
Last Modified:	05 Aug 2013 14:29
URI:	http://irep.iium.edu.my/id/eprint/30576

Actions (login required)

View Item