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Cytological and cytochemical studies on the pulmonary alveolar macrophages of the rat “Rattus rattus norvegicus albinus”

Salman, Mohammad O. and Abd, Ali H. and Al-Ani, Imad Matloub Dally and Al-Salihi, Anam R. (2013) Cytological and cytochemical studies on the pulmonary alveolar macrophages of the rat “Rattus rattus norvegicus albinus”. Annals of Microscopy, 13. pp. 28-35. ISSN 0219-2209

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Abstract

The objective of the present study was to fi nd the normal values of the cytology and cytochemistry of the Pulmonary Alveolar Macrophages (PAMs) and through the identifi cation of universal normal values. Bronchoalveolar lavage (BAL) fl uid was performed by tracheal cannulation to seventy healthy adult male rats. The percentages of differential BAL cells count were 85-90% PAMs, 5-6% lymphocytes, 3-4% granulocytes and 3-4% epithelial cells. The diameter of PAMs was ranging between (14-35μ). The mean of PAMs area was 57μ2±1.03. The mean of nucleus area was 17.6μ2±1.3. The ratio of nucleus areas to cytoplasm area was1:2. The Final Reaction Product of the ANAE was homogenous brown reddish color. It was homogenous in distribution although the distribution was regular within the area of cytoplasm and nucleus of PAMs while other BAL cells showed negative reaction. 95% of total PAMs were positive in reaction. The mean of optical density of fi nal reaction product of esteratic activity in PAMs was 1.782±0.004.

Item Type: Article (Journal)
Additional Information: 5849/30487
Uncontrolled Keywords: Pulmonary Alveolar Macrophages, Bronchoalveolar lavage, Optical density, Tracheal cannulation, Final reaction product
Subjects: Q Science > QM Human anatomy
R Medicine > RB Pathology
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Medicine > Department of Basic Medical
Depositing User: Assoc. Prof Dr.. Imad Matloub Dally Al-Ani
Date Deposited: 06 Aug 2013 09:25
Last Modified: 18 Dec 2015 10:41
URI: http://irep.iium.edu.my/id/eprint/30487

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