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Purification of antibacterial compounds from spathiphyllum cannifolium leaf

Ahmad Raus, Raha and Abdullah, Erlina and Jamal, Parveen and Jimat, Dzun Noraini (2013) Purification of antibacterial compounds from spathiphyllum cannifolium leaf. Der Pharma Chemica, 5 (1). pp. 350-357. ISSN 0975-413X

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Spathiphyllum cannifolium is an ‘araceae’ species which can be found in Malaysia for landscaping. Previous study carried out in this lab found that this plant possesses the highest antibacterial activity among 19 flowering plants screened. In the present study, purification of antibacterial compound of the plant was conducted. For purification of antibacterial compound, open column chromatography was conducted and ethyl acetate, hexane and ethanol are used as the mobile phase. The results showed that the elution of column with 80% ethyl acetate in ethanol provide fractions with the highest antibacterial activity. The fractions were analyzed with TLC plates and revealed a single spot under UV light at 0.85 Rf value. Further purification of the single spot using HPLC showed two isolated compounds at retention time of 5.53 minutes and 8.26 minutes were obtained. The compounds were suspected as flavanoids as they can be detected at wavelength 360 nm and 400 nm.

Item Type: Article (Journal)
Additional Information: 3911/29936
Uncontrolled Keywords: S. cannifolium, antibacterial activity, chromatography, HPLC
Subjects: T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Prof.Dr. Parveen Jamal
Date Deposited: 24 Apr 2013 15:35
Last Modified: 08 Dec 2016 09:28
URI: http://irep.iium.edu.my/id/eprint/29936

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