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Preservation of hippocampal ultrastructural constituents with Nigella sativa oil treatment to rats challenged by global cerebral oligemia

Azzubaidi, Marwan Saad and Talib, Norlelawati A. and Saxena, Anil Kumar and Ahmed, Qamar Uddin and Al-Ani, Imad Matloub Dally (2013) Preservation of hippocampal ultrastructural constituents with Nigella sativa oil treatment to rats challenged by global cerebral oligemia. In: IIUM Research, Invention and Innovation Exhibition (IRIIE) 2013, 19-20 Feb 2013, Cultural Activity Centre (CAC) and KAED Gallery, IIUM.

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Abstract

The oil extract of Nigella sativa seeds (NSO) was found to preserve viability of cerebellar neurons in vitro. Recent in vivo experimental study proved that NSO daily oral treatment was able to prevent memory and learning deterioration in a murine model of mild global cerebral ischemia (MGCI) that was created with permanent bilateral occlusion of the common carotid arteries (2VO) in rats. The current study’s object ive was to assess the effect of NSO treatment on pyramidal and other neighboring neurons within CA1 hippocampal subfield of rats with MGCI that was achieved through 2VO procedure. The assessment was ultrastructural using transmission electron microscopy (TEM). Nine rats were equally divided into three groups: sham control, untreated 2VO and NSO treated group (2VO with daily oral NSO treatment). By the end of the 10th postoperative week coronal sections of the hippocampus were collected and processed for transmission electron microscopy (TEM). The neurodegenerative changes observed in pyramidal cells, astrocytes, endothelial basement membrane and axoplasms of CA1 hippocampal region of 2VO group were completely absent in sham operated control group. Meanwhile, NSO treated groups were almost free of ultrastructural neurodegenerative changes as those detected in untreated 2VO group. This adds another supporting evidence of the promising neuroprotective activity provided by NSO treatment to prevent age related neurodegenerative diseases especially those associated with chronic global cerebral hypoperfusion such as Alzheimer’s disease.

Item Type: Conference or Workshop Item (Poster)
Additional Information: 6809/29175
Uncontrolled Keywords: neurodegeneration, 2VO, hippocampus, Nigella sativa, neuroprotection, rat
Subjects: Q Science > QP Physiology
R Medicine > R Medicine (General)
R Medicine > RB Pathology
R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
R Medicine > RM Therapeutics. Pharmacology > RM300 Drugs and their action
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Medicine > Department of Basic Medical
Kulliyyah of Pharmacy > Department of Basic Medical Sciences
Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User: Dr. Marwan Saad Azzubaidi
Date Deposited: 19 Aug 2013 11:07
Last Modified: 18 Dec 2015 11:02
URI: http://irep.iium.edu.my/id/eprint/29175

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