Antioxidant behaviour of carotenoids highly accumulated in HepG2 cells

Jaswir, Irwandi and Kobayashi, Miyuki and Koyama, Toshie and Kotake-Nara, Eiichi and Nagao, Akihiko (2012) Antioxidant behaviour of carotenoids highly accumulated in HepG2 cells. Food Chemistry, 132 (2). pp. 865-872. ISSN 0308-8146

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Abstract

The antioxidant behaviour of major dietary carotenoids accumulated at high concentrations in human hepatoma HepG2 cells was evaluated, in comparison with a-tocopherol. The cells that accumulated carotenoids and a-tocopherol at levels higher than the values reported in the human liver were exposed to mild oxidative stress with tert-butylhydroperoxide. b-Carotene (>2.6 nmol/mg protein) and astaxanthin (>1.8 nmol/mg protein) significantly suppressed lipid peroxidation, while b-cryptoxanthin and lutein did not. a-Tocopherol remarkably suppressed lipid peroxidation with an IC50 value of 0.16 nmol/mg protein. Neither a-tocopherol nor any of the carotenoids except for lycopene showed pro-oxidant action even at high cellular concentrations. The antioxidant behaviours of carotenoids in a cellular milieu were quite different from those previously found in liposomes and homogeneous solutions. Further studies are required to assess the implications of the antioxidant behaviours found in the cultured cells on human health.

Item Type:	Article (Journal)
Additional Information:	5784/28710
Uncontrolled Keywords:	Antioxidant; Carotenoid; HepG2 cells; Pro-oxidant; α-Tocopherol
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering
Depositing User:	Prof. Dr. Irwandi Jaswir
Date Deposited:	20 Mar 2013 09:37
Last Modified:	01 Aug 2021 22:29
URI:	http://irep.iium.edu.my/id/eprint/28710

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