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Supercritical Carbon Dioxide Extraction of Seed Oil from Winter Melon (Benincasa hispida) and Its Antioxidant Activity and Fatty Acid Composition

Bimakr, Mandana and Rahman, Russly and Taip, Farah Saleena and Mohd Adzahan, Noranizan and Sarker, Md. Zaidul Islam and Ganjloo, Ali (2013) Supercritical Carbon Dioxide Extraction of Seed Oil from Winter Melon (Benincasa hispida) and Its Antioxidant Activity and Fatty Acid Composition. Molecules, 18 (1). pp. 997-1014. ISSN 1420-3049

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Abstract

In the present study, supercritical carbon dioxide (SC-CO2) extraction of seed oil from winter melon (Benincasa hispida) was investigated. The effects of process variables namely pressure (150–300 bar), temperature (40–50 °C) and dynamic extraction time (60–120 min) on crude extraction yield (CEY) were studied through response surface methodology (RSM). The SC-CO2 extraction process was modified using ethanol (99.9%)as co-solvent. Perturbation plot revealed the significant effect of all process variables on the CEY. A central composite design (CCD) was used to optimize the process conditions to achieve maximum CEY. The optimum conditions were 244 bar pressure, 46 °C temperature and 97 min dynamic extraction time. Under these optimal conditions, the CEY was predicted to be 176.30 mg-extract/g-dried sample. The validation experiment results agreed with the predicted value. The antioxidant activity and fatty acid composition of crude oil obtained under optimized conditions were determined and compared with published results using Soxhlet extraction (SE) and ultrasound assisted extraction (UAE). It was found that the antioxidant activity of the extract obtained by SC-CO2 extraction was strongly higher than those obtained by SE and UAE. Identification of fatty acid composition using gas chromatography (GC) showed that all the extracts were rich in unsaturated fatty acids with the most being linoleic acid. In contrast, the amount of saturated fatty acids extracted by SE was higher than that extracted under optimized SC-CO2 extraction conditions.

Item Type: Article (Journal)
Additional Information: 6605/28631
Uncontrolled Keywords: winter melon (Benincasa hispida); supercritical carbon dioxide extraction; response surface methodology; fatty acid composition; antioxidant activity
Subjects: Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User: Professor Dr. Md. Zaidul Islam Sarker
Date Deposited: 18 Jan 2013 15:25
Last Modified: 24 Feb 2016 18:30
URI: http://irep.iium.edu.my/id/eprint/28631

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