Cytotoxicity study of Schizophyllum commune extract towards DF-1 cell line

Abd rahman, Nur Rahilah and Ahmad Raus, Raha and Mel, Maizirwan (2012) Cytotoxicity study of Schizophyllum commune extract towards DF-1 cell line. In: 26th Symposium of Malaysian Chemicals Engineers in conjunction with the 4th International Conference on Chemical and Bioprocess Engineering, 21-23 November 2012, Kota Kinabalu.

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Abstract

Schizophyllum commune is a filamentous fungus widely known and distributed across the world continents. Although S. commune was acknowledged for its medicinal properties in countries like China and Japan, the Malaysian strain of S. Commune has not been fully utilized and developed. These basidiomycetes can be found abundantly in rubber plantations across Malaysia especially after raining season. S. commune excreted an exopolysaccharide known as Schizophyllan which can be identified as a homoGlucan containing a β-(1,3)-linked backbone with single β-(1,6)-linked glucose side chains at every third residue. Schizophyllan is believed to have an antiviral, antimicrobial as well as anticancer activity. The purpose of this study is to observe the cytotoxicity effect of S. commune extract towards the DF-1 cell line. The selected Malaysian strain of S.commune mycelia were cultivated by liquid fermentation using shake flasks in selective media and under certain condition to produce the secondary bioactive compound. Compound was extracted by addition of isopropanol that will be separated later using centrifugation. The analysis of S. commune extract by FTIR showed the weak bond at 890 cm-1 as evidenced of the β-glycosidic linkage characteristic. The cytotoxicity analysis showed only 37 percent of inhibition rate of DF-1 cell line viability for 1 mg/ml of S. commune extract and the value of IC50 is higher 100 mg/ml showed there was no cytotoxic effect. Therefore, the S. commune is safe to be used for further study involving this cell line.

Item Type:	Conference or Workshop Item (Full Paper)
Additional Information:	ISBN No 978-983-2641-95-7, 4085/27360
Uncontrolled Keywords:	CYTOTOXICITY,SCHIZOPHYLLUM COMMUNE, EXTRACT DF-1 CELL LINE
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology T Technology > TP Chemical technology > TP500 Fermentation industries. Beverages. Alcohol
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr Maizirwan Mel
Date Deposited:	10 Dec 2012 21:54
Last Modified:	14 Dec 2012 14:17
URI:	http://irep.iium.edu.my/id/eprint/27360

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