Ultrastructural assessment of the CA1 hippocampal neuronal changes in curry leaves extract treated rats subjected to chronic cerebral hypoperfusion

Azzubaidi, Marwan Saad and Mohd Noor, Noriah and A.Talib, Norlelawati and Saxena, Anil Kumar and Al-Ani, Imad Matloub Dally and Maideen, Mohd Maizam (2012) Ultrastructural assessment of the CA1 hippocampal neuronal changes in curry leaves extract treated rats subjected to chronic cerebral hypoperfusion. In: 21st Scientific Conference of the Microscopy Society Malaysia, 22nd-24th November 2012, Renaissance Hotel, Kota Bharu, Kelantan, Malaysia.

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Abstract

Curry leaves VIZ. Murraya koenigii leaves (MKL) extract was found to possess robust antioxidant and anticholinesterase activities. Its memory enhancing effect in preclinical studies has also been reported. The current study was designed to evaluate the ultrastructural neurodegenerative changes within CA1 subfield of the hippocampus that result from chronic cerebral hypoperfusion (CCH) in rats, and to assess the possible preventive potential of MKL treatment to these changes. Rats were divided into sham-control, CCH and CCH+MKL treated groups. Ten weeks after surgical induction of CCH rats’ hippocampal sections were processed for transmission electron microscopical examination of CA1 subfield. Sham-control group exhibited normal pyramidal neurons with intact nuclei and abundant cytoplasmic organelles. CCH group hippocampi showed a mixture of necrotic and apoptotic neurodegeneration of pyramidal neurons with extensive loss of cytoplasmic organelles. Thickening of the endothelial basement membrane was observed with evident hypertrophy of astrocytic processes which constitute the vascular end-feet. CCH+MKL treated group demonstrated predominant apoptotic neurodegeneration. The pyramidal cells’ nuclei were shrunken with occasional formation of apoptotic bodies. Distorted intracellular organelles and abnormally thickened capillary endothelial basement membrane was noticed too. In conclusion, MKL treatment could not protect the CA1 hippocampal subfield from the CCH induced ultrastructural neurodegenerative changes.

Item Type:	Conference or Workshop Item (Full Paper)
Additional Information:	6809/27084
Uncontrolled Keywords:	CA1 hippocampal neural changes
Subjects:	R Medicine > RB Pathology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Medicine > Department of Basic Medical Kulliyyah of Pharmacy > Department of Basic Medical Sciences
Depositing User:	Dr. Marwan Saad Azzubaidi
Date Deposited:	27 Nov 2012 08:16
Last Modified:	18 Dec 2015 11:02
URI:	http://irep.iium.edu.my/id/eprint/27084

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