Anti-cancer activities of Thymoquinone, constituents of Nigella sativa against oral cancer cell lines

Arief Ichwan, Solachuddin Jauhari and Taher, Muhammad and Masa-Aki , Ikeda and Z. L, Abdullah and M.T, Junaidi (2010) Anti-cancer activities of Thymoquinone, constituents of Nigella sativa against oral cancer cell lines. In: 9th Scientific Meeting of International Association for Dental Reesarch, 5-6 February 2010, UKM, Kuala Lumpur. (Unpublished)

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Abstract

Objectives: The aims of this study were to investigate and determine anti-cancer potential of Thymoquinone (TQ), a major component of Nigella Sativa seeds against cell lines derived from oral cavity carcinoma. Methods: Three human cancer cell lines derived from oral cavity, Ca922, HSC-2 and HSC-3 were used. HCT116 (human colorectal carcinoma) cells were also used as the positive control. The cells were exposed to TQ solution at different concentrations and then analyzed after certain culture periods (12 and 24 hours). Cytotoxic activity was quantified by MTT assay. Apoptosis (programmed cell death) induction was detected by DNA Fragmentation assay. Wound-healing migration assay (Scratch assay) was performed to determine cells migration. Results: TQ dose- and time-dependently reduced the viability of Ca922, HSC-2 and HSC-3 cells. TQ treatment at 20 μM was sufficient to inhibit cancer cell migration after 12 hours. Moreover, DNA fragmentation assay shows that TQ treatment induced apoptosis in all of cell lines tested. Conclusions: TQ exhibits anti-cancer activities against oral cancer cell lines. This study suggests the potential benefit of TQ as a natural chemotherapeutic agent for oral cancer treatment.

Item Type:	Conference or Workshop Item (Speech/Talk)
Additional Information:	5731/23822
Uncontrolled Keywords:	Thymoquinone,Nigella sativa, oral cancer
Subjects:	R Medicine > RK Dentistry R Medicine > RM Therapeutics. Pharmacology > RM300 Drugs and their action
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Dentistry Kulliyyah of Pharmacy
Depositing User:	Dr. Solachuddin J.A. Ichwan
Date Deposited:	31 Dec 2012 15:21
Last Modified:	21 Mar 2013 14:31
URI:	http://irep.iium.edu.my/id/eprint/23822

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