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Recombinant bromelain production in Escherichia coli: process optimization in shake flask culture by response surface methodology

Muntari, Bala and Amid, Azura and Jami, Mohammed Saedi and Mohd. Salleh, Hamzah and Mel, Maizirwan (2012) Recombinant bromelain production in Escherichia coli: process optimization in shake flask culture by response surface methodology. AMB Express, 2 (12). pp. 1-9. ISSN 2191-0855

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Abstract

Bromelain, a cysteine protease with various therapeutic and industrial applications, was expressed in Escherichia coli, BL21-AI clone, under different cultivation conditions (post-induction temperature, L-arabinose concentration and post-induction period). The optimized conditions by response surface methodology using face centered central composite design were 0.2% (w/v) L-arabinose, 8 hr and 25°C. The analysis of variance coupled with larger value of R2 (0.989) showed that the quadratic model used for the prediction was highly significant (p < 0.05). Under the optimized conditions, the model produced bromelain activity of 9.2 U/mg while validation experiments gave bromelain activity of 9.6 ± 0.02 U/mg at 0.15% (w/v) L-arabinose, 8 hr and 27°C. This study had innovatively developed cultivation conditions for better production of recombinant bromelain in shake flask culture.

Item Type: Article (Journal)
Additional Information: 3688/23637
Uncontrolled Keywords: bromelain; Escherichia coli BL21-AI; face centered central composite design; induction
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr Azura Amid
Date Deposited: 12 Jun 2012 11:04
Last Modified: 17 Jul 2014 09:37
URI: http://irep.iium.edu.my/id/eprint/23637

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