Muntari, Bala and Amid, Azura and Jami, Mohammed Saedi and Mohd. Salleh, Hamzah and Mel, Maizirwan (2012) Recombinant bromelain production in Escherichia coli: process optimization in shake flask culture by response surface methodology. AMB Express, 2 (12). pp. 1-9. ISSN 2191-0855
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Abstract
Bromelain, a cysteine protease with various therapeutic and industrial applications, was expressed in Escherichia coli, BL21-AI clone, under different cultivation conditions (post-induction temperature, L-arabinose concentration and post-induction period). The optimized conditions by response surface methodology using face centered central composite design were 0.2% (w/v) L-arabinose, 8 hr and 25°C. The analysis of variance coupled with larger value of R2 (0.989) showed that the quadratic model used for the prediction was highly significant (p < 0.05). Under the optimized conditions, the model produced bromelain activity of 9.2 U/mg while validation experiments gave bromelain activity of 9.6 ± 0.02 U/mg at 0.15% (w/v) L-arabinose, 8 hr and 27°C. This study had innovatively developed cultivation conditions for better production of recombinant bromelain in shake flask culture.
Item Type: | Article (Journal) |
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Additional Information: | 3688/23637 |
Uncontrolled Keywords: | bromelain; Escherichia coli BL21-AI; face centered central composite design; induction |
Subjects: | Q Science > Q Science (General) Q Science > QR Microbiology |
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): | Kulliyyah of Engineering > Department of Biotechnology Engineering |
Depositing User: | Prof Ts Dr Azura Amid |
Date Deposited: | 12 Jun 2012 11:04 |
Last Modified: | 14 Jul 2021 10:18 |
URI: | http://irep.iium.edu.my/id/eprint/23637 |
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