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Effect of homogenization in breaking protein-carotenoid complexes for releasing active compounds

Jamal, Parveen and Jaswir, Irwandi and Hashim, Nurhasri Mulyadi and Azmi, Saiful Mohammad Nizam (2011) Effect of homogenization in breaking protein-carotenoid complexes for releasing active compounds. In: Current research and development in biotechnology engineering at IIUM. IIUM Press, Kuala Lumpur, pp. 285-295. ISBN 9789674181444

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Abstract

The effect of homogenization in breaking protein-carotenoid complexes from cassava leaves was examined by monitoring three process conditions; amount of solvent per 0.5 g sample (ml), homogenization speed (rpm) and homogenization time (s). Acetone-n-hexane (1:1, v/v), tetrahydrofuran-methanol (1:1 v/v) and acetone were employed in the extraction process. The solvent that exhibited the best characteristics in extracting the highest b-carotene yield from the sample was selected for the optimizatIOn of process conditions for maximum b-carotene concentration, utilizing Face Centered Central Composite Design (FCCD) from Design Expert software, version 6 (DX6). A total of 20 experiments comprised of three factors, three levels and 6 center points were conducted to facilitate the optimization process. The maximum b-carotene yield of 17,544 flg/lOO g sample was achieved with the solvent ratio of 12 ml to 0.5 g sample, homogenization speed of 22000 rpm and homogemzation time of 57 s.

Item Type: Book Chapter
Additional Information: 2937/20992
Uncontrolled Keywords: Protein-Carotenoid Complexes, b-carotene, Cassava Leaves, Homogenization, Face Centered Central Composite Design
Subjects: T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Prof.Dr. Parveen Jamal
Date Deposited: 05 Jul 2012 12:12
Last Modified: 05 Jul 2012 12:13
URI: http://irep.iium.edu.my/id/eprint/20992

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