Abdul Karim, Mohamed Ismail and A. Hamid, Manisya Zauri and Yusof, Faridah and Alam, Md. Zahangir
(2011)
Screening of fungi on solid state bioconversion of oil palm empty fruit bunch for production of cellulase.
In:
Current research and development in biotechnology engineering at IIUM.
IIUM Press, Kuala Lumpur, pp. 251-257.
ISBN 9789674181444
Abstract
Five potential strain of fungi (Trichoderma reesei RUT C-30 (ATCC 56765, USA;
Trichoderma harzanarium (SCahmT 105); Trichoderma harzianum (Isolated by UPM);
Penicillium 1 (isolated from sewage treatment plant);Peniczllium 2 (isolated from empty fruit
bunch) were screened for production of cellulase using solid state bioconversIOn of oil palm
empty fruit bunch. Hydrolytic activities of strains were tested on carboxymethylcellulose
(CMC) agar. Strain Penicillzum 2 (P 2) showed the highest radius of hydrolytic activity of 1.2
cm. This was followed by T. reesez RUT C-30 (RUT C-30) and isolated Peniczllium 1 (P 1)
with radius clearance of 1.0 and 0.9 cm, respectively. These strains of culture (Ix 107
spores/ml ) were seeded in oil palm empty fruit bunch (70% moisture) supplemented with 1N
of potassium dihydrogen phosphate and 1N of potassium hydrogen phosphate in shake flask
experiment which was fermented for 8 days at 30°C for production of cellulase. Result
obtained indicate T. reesei RUT C-30 produce the highest CMCase (1.831 U/gds) at day 6
of fermentation. For FPase Trichoderma harzianum gave high production (0.156 U/gds at 4
days fermentation. Penicillium 1 produced the highest ~-glucosidase of 2.361 U/gds at day
2fermentation.
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