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A study of bacterial cell immobilization in alginate gel beads for the production of Myo-Inositol Phosphates

Mohamad Puad, Noor Illi and Farouk, Abd Alaziem and Mohd. Salleh, Hamzah (2011) A study of bacterial cell immobilization in alginate gel beads for the production of Myo-Inositol Phosphates. In: Current Research and Development in Biotechnology Engineering at IIUM (volume II). IIUM Press, Kuala Lumpur, pp. 36-42. ISBN 978-967-418-151-2

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Abstract

A no\'el phyrale-degrnding enzyme namely PhyFAUIA I was purificd from a bacTerium i~lated from Malaysian wastewatcr. It is a pcriplasmaTic cnzymc and highly substrate-specific to phylaTe. The bacTerial cells were immobilized in alginate gel beads and iTS condition for preparaTion of myo-inositol phosphatcs was invcsligaled. The conditions for immobilizcd eclls were opTimized using dilTerem mcdia, residence Time and Temperature. Upon immob,lizalion. beads containing cells were sTored in groWlh medium at 4°C and Thc yicld can be reTained for more than 24 hours. The mechanism of reaction beTween phylaTe and PhyFAUIAI using immobilizcd cells was also invcsTigated and thcre were 2 possible paThways: (II PhyFAUlAl was seereted from the cells and went OUI from the beads to react Wilh phylatc. (2) PhyTate diffused Through lhc pores of alginate beads and reacTed WiTh phytasc locatcd inside the beads as well as aT The pcriplasmic OfThc cells. P, and IP were Then released from the beads.

Item Type: Book Chapter
Additional Information: 3688/20702
Uncontrolled Keywords: Cell immobilizationn. alginate. phytate degrading enzyme, myo-inositol phosphates
Subjects: T Technology > TP Chemical technology
T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr Hamzah Mohd. Salleh
Date Deposited: 27 Nov 2012 15:27
Last Modified: 27 Nov 2012 15:29
URI: http://irep.iium.edu.my/id/eprint/20702

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