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Purification of serine protease from mango (Mangifera Indica Cv. Chokanan)peel using an alcohol/salt aqueous two phase system

Sarker, Md. Zaidul Islam and Amid, Mehrnoush and Shuhaimi, Mustafa and Abdul Manap, Mohd Yazid (2012) Purification of serine protease from mango (Mangifera Indica Cv. Chokanan)peel using an alcohol/salt aqueous two phase system. Food Chemistry, 132 (3). pp. 1382-1386. ISSN 0308-8146 (In Press)

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Abstract

An alcohol/salt-based aqueous two-phase (ATPS) system, as a novel method of purification, was employed to purify serine proteases from mango (Mangifera Indica Cv. Chokanan) peel. The effectiveness of different parameters, such as type and concentration of alcohol (1-propanol, 2-propanol, and ethanol), type of salt (sodium citrate, potassium phosphate, and ammonium sulphate), pH, and NaCl, on the purification and selective separation of serine protease was investigated. Desirable conditions of partition coefficient (K), selectivity (S), purification factor (P), and yield (Y%) of serine protease, using ATPS, were determined. The highest partition coefficient (64.5) and selectivity (343.2) for serine protease purification value were achieved in an ATPS of 16% (w/w) 2-propanaol, 19% (w/w) potassium phosphate, and 5% (w/v) NaCl at pH 7.5. It was demonstrated that serine protease could be recovered with a yield of 96.7% and a purification factor of 11.6.

Item Type: Article (Journal)
Additional Information: 6605/17913
Uncontrolled Keywords: purification, alcohol/salt system, serine protease, mango peel, purification factor, yield
Subjects: Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User: Professor Dr. Md. Zaidul Islam Sarker
Date Deposited: 09 Jan 2013 14:17
Last Modified: 24 Feb 2016 16:15
URI: http://irep.iium.edu.my/id/eprint/17913

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